Primary culture of fibroblast cells from rat/mouse
Posted 11 April 2013 - 12:13 PM
Does anyone have the protocol/paper to culture primary fibroblast cells from the tail or whatever sources of organs/tissues from rat and mouse.
Posted 11 April 2013 - 12:26 PM
Take tissue sample, sterilize surface in bleach (10s) then rinse in sterile water and rinse with 70% ethanol.
Mince tissue into small chunks using sterile scapels (may help to put softer tissues through a cell strainer).
Digest chunks with a protease for some time (tissue dependent).
Neutralize protease and plate out.
Posted 15 April 2013 - 06:12 PM
Posted 15 April 2013 - 09:07 PM
Posted 16 April 2013 - 03:09 AM
Thanks alot for the suggestion...Maybe gotta optimise own protocol