Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Transfection/Infection Problems


  • Please log in to reply
1 reply to this topic

#1 Chris Netherton

Chris Netherton

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 February 2004 - 02:59 AM

Hello!

I was wondering if anyone has any tips/advice on transfection/infection experiments.
We're currently using Promega's TransFast transfection reagent, but are having problems finding transfected and infected cells. i.e., the cross-over between the two is very poor. TransFast seems to stick to coverslips, (and, unsurprisingly the cells!). This binds DAPI when we do immunofluorescence experiments and we're wondering if this is inhibiting the subsequent infection. Unfortunately, we're limited by the rate of viral replication, so we can't infect then transfect!
Does anyone have any transfection reagents they'd recommend for this type of work.

Cheers

Chris

#2 Skeletor

Skeletor

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 18 February 2004 - 09:17 AM

What cells are you working with?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.