RT-PCR product- no band
Posted 11 April 2013 - 02:50 AM
I'm working on Grapevine Leafroll Associated Viruses. I'm using a multiplex PCR protocol, but it doesn't work, even the positive controls. What could be the problem? If you have ant idea about this, please inform me. Thanks in advance.
Posted 11 April 2013 - 07:08 AM
Posted 11 April 2013 - 11:41 PM
Posted 12 April 2013 - 07:37 AM
Make sure your reagents are good. I assume you have used these reagents (minus the primers) successfully with something else. If not, test your reagents with template and primer that have had positive results in your hands or in your lab.
Beyond that, especially if you designed the primers yourself, make sure each primer set works by itself before combining multiple primer sets in the reaction.
Posted 15 April 2013 - 02:57 AM