Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Plasmid Extraction from P. Aeruginosa smears in Gel!

plasmid extraction pa14 smear gel

  • Please log in to reply
2 replies to this topic

#1 tihong10



  • Active Members
  • PipPipPipPipPip
  • 52 posts

Posted 11 April 2013 - 01:13 AM

Hi, I electroporated P. aeruginosa 14 with a plasmid and when I tried to view the plasmid extraction in a gel it came out as a smear. Is there some big issue I am not aware of when plasmid extracting from PA? Is my plasmid being degraded somehow?

I wasn't sure how much detail to put but if more is needed I will provide it.


#2 pito



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,474 posts

Posted 12 April 2013 - 04:00 AM

could be so many things...
perhaps putting a picture of the gel here could help?

it could be that you somehow broke the plasmid or that you are seeing non plasmid dna...

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.

#3 tihong10



  • Active Members
  • PipPipPipPipPip
  • 52 posts

Posted 16 July 2013 - 11:48 PM


I wanted to bump this post back up...

Here is a gel image of the P. aeruginosa 14 (transformant containing a recombinant plasmid) plasmid extraction that I performed using a Plasmid miniprep kit (using the optional denaturation buffer).

Whenever I perform a P.E. on PA14 I always get this kind of smear. Can anyone shed some light on this smearing pattern that keeps appearing and possibly a way I can cleanly extract a plasmid from the PA14 bacteria?

Thank you,

Also tagged with one or more of these keywords: plasmid extraction, pa14, smear, gel

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.