3 replies to this topic

[Mad researcher]

Hello,

could you please suggest me some software/excel sheet/formula to calculate normlaization factor for qPCR data?

[cellthetruth]

Maybe this helps:

A new mathematical model for relative quantification in real-time RT-PCR.
Pfaffl MW.
Nucleic Acids Res. 2001 May 1;29(9):e45

[Tabaluga]

On this (by the way excellent) real time pcr tutorial website you can find the link to an excel sheet using the Pfaffl Method:
http://pathmicro.med.../pcr-pfaffl.htm

And here is the tutorial itself:
http://pathmicro.med...altime-home.htm

Edited by Tabaluga, 09 April 2013 - 06:29 AM.

[Mad researcher]

Thanks guys