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Normalization factor


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3 replies to this topic

#1 Mad researcher

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Posted 06 April 2013 - 07:10 AM

Hello,

could you please suggest me some software/excel sheet/formula to calculate normlaization factor for qPCR data?
Cheers,

Mad Researcher

#2 cellthetruth

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Posted 08 April 2013 - 04:58 AM

Maybe this helps:

A new mathematical model for relative quantification in real-time RT-PCR.
Pfaffl MW.
Nucleic Acids Res. 2001 May 1;29(9):e45

#3 Tabaluga

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Posted 09 April 2013 - 06:27 AM

On this (by the way excellent) real time pcr tutorial website you can find the link to an excel sheet using the Pfaffl Method:
http://pathmicro.med.../pcr-pfaffl.htm

And here is the tutorial itself:
http://pathmicro.med...altime-home.htm

Edited by Tabaluga, 09 April 2013 - 06:29 AM.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#4 Mad researcher

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Posted 09 April 2013 - 07:43 AM

Thanks guys :)
Cheers,

Mad Researcher




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