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Addition of antibiotic to the culture medium


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#1 Mohamed samir

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Posted 05 April 2013 - 11:27 AM

I gave some questions regarding cell culture
1. Is it benificial or harmful to add antibiotic with the known conc 1 % to my medium ? some people claim that it is better not to add antibiotic to ur medium if u have sterile experiment.
2. what are the disadvantages of this addition ?
3. I know that it is better not to add antibiotic to primary cells. why especially primary cell ?


Thanks

#2 Tabaluga

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Posted 05 April 2013 - 03:29 PM

Well, a disadvantage is for instance that low level infections are suppressed by the antibiotic but could actually still effect your experiments.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 





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