I am trying to do purification of a protein having MBP tag. After sonication, i am seeing a band of 42 kDa and suspecting it to be MBP breakdown product. After extensive washing with column buffer i eluted the protein with 10mM maltose and agian i am seeing multiple bands. I need to purify the protein to homogeneity for my activity assay. Please anybody who overcame this help me.
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MBP-Tagged protein degrading after expression
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