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Antibody detection problem

Antibody blotting

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#1 Waqar Ahmad

Waqar Ahmad

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Posted 03 April 2013 - 05:10 AM

Dear Fellows

I am using monoclonal anti amyloid beta anitibody for amyloid beta detection in C. elegans. After running gel/ western it gives band at 43 KDa, however studies showed that this band should be at 4-20 kDa. Please advise me what to do? Secondly I am not getting 4 KDa band although I used 15% SDS gel

Regards
Waqar

#2 mdfenko

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Posted 03 April 2013 - 06:21 AM

amyloid beta (especially 1-42) readily aggregates. you may be detecting that or you may be detecting amyloid precursor protein.

for 4kDa, i would use tris-tricine (shagger & von jagow) page.

here is an archived post with the formulation.
talent does what it can
genius does what it must
i do what i get paid to do





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