How to prevent albumin masking of urokinase from conditioned media
Posted 03 April 2013 - 04:44 AM
However, albumin is complicating things because he dominates in my conditioned media extracts and I am not getting any uPA bands on Western blot, and when I Amidoblack stain my PVDF membranes I can clearly see one big albumin band from 45-65 kDA.
I am cultivating cells prior to extracting conditioned media, 6 hrs in serum-free media. Should I increase that time to get rid of albumin or do you know any other way how to prevent albumin interfering with my urokinase identification. Much obliged
Posted 03 April 2013 - 06:39 AM
here are a few of the available kits:
[url="http://www.emdmillipore.com/life-science-research/proteoextract-albumin-removal-kit/EMD_BIO-122640/p_xdCb.s1ORVMAAAEjpxp9.zLX"]calbiochem (emd millipore)[/url]
- Josip Madunic likes this
genius does what it must
i do what i get paid to do
Posted 04 April 2013 - 01:41 AM
Edited by Josip Madunic, 04 April 2013 - 01:42 AM.