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MTT assay trouble shooting

Mtt

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#1 Gunalan

Gunalan

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Posted 02 April 2013 - 08:17 AM

Hi
I performed MTT assay in mouse spleenocytes .... I am getting colour development even in RPMI medium alone wells where in no cell is there
what could be the possible problem.

how to trouble shoot the problem.

I prepared RPMI1640 without phenol red from Sigma according tot eh directions and added FBS to it, filtered and employed it for the assay.

MTT was prepared in PBS

#2 antitoxin

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Posted 16 October 2013 - 01:24 PM

with hello 

i think mtt prepare in Distilled water 







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