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Immunofluorescence - Fixing Cells Overnight


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#1 Debbie_M

Debbie_M

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Posted 02 April 2013 - 04:21 AM

Hey everyone,

I am currently setting up a time course experiment where I will be making microscopy slides every hour for 12 hours and I then want to do some immunofluorescence with the slides. For the standard protocol we use, we fix cells in 70% Ethanol for 30 minutes but because I want to do so many slides it would be good if I could keep them all until the next day before I do the staining protocol (which takes several hours).

Does anyone know if the cells on coverslips will be OK overnight in 70% ethanol? or is there a better point at which I should stop the experiment so I can get some sleep?

I would appreciate any advice, I dread setting up the experiment and the fixing overnight causes an issue!

Thanks,

Debbie




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