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GFP -weak signal -why?

GFP weak signal cloning

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#1 Louise Von Gersdorff Jørg

Louise Von Gersdorff Jørg

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Posted 01 April 2013 - 11:49 PM

Hello :)
I have cloned various inserts into a GFP vector. I can still see the GFP after the successful clonings however the signal is much weaker compared to the original GFP vector. I need full strength of the GFP. What do I do?

Insert a spacer region between the GFP and my DNA sequence? Is it possible that my insert somehow inhibits the GFP signal?

Please help :)

Best regards,
Louise

#2 bob1

bob1

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Posted 02 April 2013 - 12:54 AM

Could be that the folding of the finished protein alters the GFP structure and hence fluorescence. It could also be that the constructs don't transfect as well as the "empty" vector.





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