Some primers dose not work
Posted 31 March 2013 - 02:29 PM
Posted 31 March 2013 - 02:53 PM
Posted 01 April 2013 - 01:57 AM
Also, are this newly designed primers (ie. never tested) or have they been shown to work before?
The more you can tell us (detailed protocols you've used), the better we can help you.
Posted 01 April 2013 - 08:54 AM
The protocol, he looks for the expression level of iRG1 gene in the A549 cell line. He ordered the primers without designing them . the primers came directly from the company.
Posted 03 April 2013 - 09:18 AM
Then check on the gel: Is the band in the correct size ? If yes, at which temperature did it work best ?
I think almost a doctor meant the detailed PCR protocol (how much of which reagent etc.)
What do you mean by "he ordered the primers without designing them" ? Of course they came from the company, but he must either have looked the sequence up in some publication where they were used or designed them via PrimerBLAST or Primer3 etc. for the company to synthesize them...
Posted 04 April 2013 - 04:39 AM
genius does what it must
i do what i get paid to do