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Truncated gene Cloning


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#1 Mariam

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Posted 29 March 2013 - 07:02 AM

Hi,
I am going to clone the truncated form of my gene of interest in pcDNA vector. Since the truncated form has not been designed from the beginning of the gene, I do not know if I should insert ATG start codon at the beginning of a gene sequence?

#2 phage434

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Posted 30 March 2013 - 06:28 AM

Yes.




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