Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Protein quantification using HPLC

  • Please log in to reply
1 reply to this topic

#1 Ubiquitous



  • Active Members
  • Pip
  • 14 posts

Posted 27 March 2013 - 10:53 AM

Does anyone have a good reference w/ a protocol for using HPLC and a standard curve to quantify protein levels in a cell lysate? My antibody seems to be terrible for performing a western blot, I wonder if it will work better to IP out the protein then detect it using HPLC? I can buy the protein in question from a vendor, and assume just make a standard curve, measure peak area via HPLC, come up with a concentration standard curve.

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,363 posts

Posted 28 March 2013 - 04:45 AM

that should work. the software that comes with some hplcs allow you to identify and quantitate the peaks. just make sure that the peaks are well separated and reproducible.

talent does what it can
genius does what it must
i used to do what i got paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.