My first question concerns when a 'primary cell culture' becomes a 'cell line'? To clarify our definitions for these terms:
- a 'primary cell culture' is a cell culture comprised of cells expanded directly from living tissue prior to being passaged
- a 'cell line' is a cell culture comprised of a stable and homogeneous population of cells of common biological origin, derived through the passaging of a primary cell culture or the alteration of an existing cell line (e.g. a stable genetic alteration)
If you are a biologist with experience establishing or using cell cultures and lines, your feedback here would be much appreciated. We are trying here to focus on defining the concepts and not getting hung up on their labels - so keep this in mind as you reply. Thanks everyone!
Edited by mhb120, 27 March 2013 - 09:37 AM.