Western blotting transferdistorted ladder bands
Posted 27 March 2013 - 09:04 AM
I am regularly using western blotting technique and often I get distorted (ladder and /or protein) bands after the western blot transfer, though there is no band distortion during the gel run.
I read that it could be due to air bubbles and inadequate uptake of buffer by the filter paper. I noticed no trapped air bubbles each time. I normally do 2 - 4 blots at one time and some of them show the distorted bands but not all of them.
I cant figure out what is the exact problem. Could you please suggest possible reasons and how to overcome to these problems?
Posted 28 March 2013 - 04:33 AM
Edit: Are you using pre-made gels or are you making your own?
Edited by jerryshelly1, 28 March 2013 - 04:34 AM.
Posted 29 March 2013 - 12:11 PM