cell Transfection and western blot
Posted 12 February 2004 - 05:45 AM
Posted 24 February 2004 - 01:11 PM
Posted 25 February 2004 - 11:59 PM
You should start by crossing out what is not wrong:
Have you ever seen your protein being expressed (by this particular construct (by any other constuct, has anybody overexpressed it before))?
Does the DNA look fine on gel?
Both OK? > then the DNA is probably OK.
What is different between when you did see it and your current experiment?
Then check the DNA carefully. Sequence to check the correct reading frame etc.
If you post what you are doing (cotransfection or not etc), what you have found so far (what worked and what didn't or any other info) your prayers will be answered with the help of this forum, i'm sure.
PS i'm praying for sun shine instead.