Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What is NEB's CutSmart Buffer?

molecular biology enzymes buffers poo poo-diddle

  • Please log in to reply
5 replies to this topic

#1 jerryshelly1

jerryshelly1

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
29
Excellent

Posted 26 March 2013 - 07:43 PM

Not really sure if this belongs here. It just seems like this CutSmart buffer appeared over night. Is this a replacement for the EcoRI buffer? Has anyone used it? It is reporting 100% activity in a double digest that NEB previously showed incompatible (my previous reactions). Can anyone confirm this?

#2 ff11

ff11

    member

  • Members
  • Pip
  • 2 posts
1
Neutral

Posted 27 March 2013 - 06:11 AM

Jerry, I'm not sure what the CutSmart buffer is made of, but "super buffers" can be very effective and have been around for a long time. An example is Mike McClelland's old KGB (Potassium Glutamate) buffer: http://www.ncbi.nlm....C334647/?page=1

#3 jerryshelly1

jerryshelly1

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
29
Excellent

Posted 27 March 2013 - 07:10 AM

That is interesting. I am glad that info is not proprietary. I am going to try this in some of my non-compatible enzyme digestions. Thanks for the link.

#4 Ahrenhase

Ahrenhase

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 161 posts
10
Good

Posted 27 March 2013 - 05:19 PM

It's buffer 4 with BSA added, I suspect

#5 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,410 posts
234
Excellent

Posted 27 March 2013 - 07:28 PM

Exactly. The composition is on their web site, and it is buffer 4 + BSA.

#6 jerryshelly1

jerryshelly1

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
29
Excellent

Posted 28 March 2013 - 04:23 AM

Ahh. I should have done more research. Thanks everyone. I guess it could be slightly beneficial.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.