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Expanding HIV-1 in U-937 Cells

HIV U-937 HIV stock moncytes/macrophages

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#1 MycoZ

MycoZ

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Posted 26 March 2013 - 02:43 PM

Hello,

I need to infect U-937 cells, differentiated with PMA into monocyte-derived-macrophages, with HIV-1. I was thinking I need to work with an "M-tropic" or "Dual tropic" strain of HIV-1 (I will use a lab adapted strain). My understanding is that when I receive the vial of HIV-1 from the source I order it from, I have to expand the stock and freeze down multiple aliquots of HIV-1 for future use. Since my lab does not work with human PBMCs, I wanted to use differentiated U-937 cells to grow up my HIV-1 viral stock.

Has anyone done this before? Is this something I can do with U-937 cells differentiated into macrophages? Papers report that U-937 cells are susceptible to infection, either with T-tropic strains before differentation or M-tropic strains after differentation. However, most protocols for HIV-1 expansion talk about using human PBMCs but since my lab doesn't work with those I was hoping to get around it. (We don't have IRB approval for that right now).

Thanks!





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