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Buffers RNase decontamination

RNases elimination

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#1 cecininí

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Posted 26 March 2013 - 10:38 AM

Hello,

I'm trying to prepare RNAlater in the laboratory. This buffer is a solution of EDTA, sodium citrate and ammonium sulfate. Must be brought to pH 5.2. The question is: I have nuclease-free water for its preparation, but the reagents are not. What is the risk to preserve my RNA In ​​this coditions or should I buy nulcease-free salts?

Thank you

#2 bob1

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Posted 26 March 2013 - 11:28 AM

You should buy nuclease free salts and only use them for RNA work, you should also never use a spatula to get these out of the container (just tip and tap the container to get them out) and never return to the container any excess you tip out.




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