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the storage time for primers


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9 replies to this topic

#1 xxcici

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Posted 26 March 2013 - 07:52 AM

How long time can primer be stored in the freezer at - 20 C? I got my primers last July and worried about that if I can still use that to get the same sensitive result.

#2 hobglobin

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Posted 26 March 2013 - 08:15 AM

Without malfunction of the freezer or many freeze-thaw cycles, primers should be working even after many years. I use primers that I ordered 7 years ago without problems (actually diluted aliquots that I prepared at that time and froze too). If there's a change in sensitivity I don't know.

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#3 phage434

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Posted 26 March 2013 - 04:27 PM

If they are in TE, they last essentially indefinitely, even at room temperature.

#4 xxcici

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Posted 27 March 2013 - 09:21 PM

thanks. I will try one batch of my experiment tomorrow. I diluted primer in water.

#5 Tabaluga

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Posted 28 March 2013 - 06:55 AM

I also store mine in water and it should be fine after this time. Can't you repeat a sample you used back then now so you can be absolutely sure about the sensitivity ?

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#6 Trof

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Posted 08 April 2013 - 11:02 AM

I was using stock batches of primers many years old.
But also, one time the stock diluted only in water, kept in -20 all the time, thawed only few times.. got completely degraded.
I use only 10mM Tris pH8 to dilute primers since.

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#7 sikander

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Posted 23 April 2013 - 07:26 PM

Hii all,

i wanna sequence my PCR product. Company person said that i need special primer for that. But i think my primer should be working for the sequencing.

Kindly help me with this.

Do i really need sequencing primer for that.
thanks

#8 pcrman

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Posted 23 April 2013 - 09:40 PM

We reconstitute primers in TE buffer to 100 uM concentration for long term storage, and prepare 10 uM working solution using water.

#9 pcrman

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Posted 23 April 2013 - 09:42 PM

Hii all,

i wanna sequence my PCR product. Company person said that i need special primer for that. But i think my primer should be working for the sequencing.

Kindly help me with this.

Do i really need sequencing primer for that.
thanks


Yes, you can use your PCR primers for sequencing reaction. Why did the company suggest you use other primers since it is PCR product which is not in a vector.

#10 sikander

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Posted 24 April 2013 - 05:22 AM

thanks PCRman for the information

i would talk to them
tc




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