3 replies to this topic

[lschier]

Hi!

I gonna start to work with co-IP (Pierce), but I never did this. I was reading about it, but it seems so confusing! I'm working with 2 cytoplasmatic proteins and co-transfecting using HEK 293. Which is the best positive and negative control in this case?

Thank you very much!!!

[bob1]

Controls could be no antibody, non-specific (i.e. not one you expect to show interaction), serum from the animal(s) the antibodies were raised in, transfected and untransfected cells...

[lschier]

Thanks, Bob!

[HOYAJM]

Just to add one more, if you have these genes knocked out in a particular genotype, antibody treatment with these make for great negative controls. I also believe for this type of work, knockdown controls are also useful with siRNA