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Controls for co-immunoprecipitation


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3 replies to this topic

#1 Lfs

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Posted 25 March 2013 - 03:00 PM

Hi!

I gonna start to work with co-IP (Pierce), but I never did this. I was reading about it, but it seems so confusing! I'm working with 2 cytoplasmatic proteins and co-transfecting using HEK 293. Which is the best positive and negative control in this case?

Thank you very much!!!

#2 bob1

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Posted 26 March 2013 - 12:38 AM

Controls could be no antibody, non-specific (i.e. not one you expect to show interaction), serum from the animal(s) the antibodies were raised in, transfected and untransfected cells...

#3 Lfs

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Posted 26 March 2013 - 09:25 AM

Thanks, Bob!

#4 HOYAJM

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Posted 27 March 2013 - 12:33 PM

Just to add one more, if you have these genes knocked out in a particular genotype, antibody treatment with these make for great negative controls. I also believe for this type of work, knockdown controls are also useful with siRNA




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