can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR
Posted 25 March 2013 - 08:38 AM
We have some canine COL1A1 primers left from a previous experiment. can I use it to measure human COL1A1 cDNA in a qPCR setup?
Posted 25 March 2013 - 09:17 AM
Quicker way to do it is to BLAST these primers against human reference transcripts.
Personally I think there is high chance the primers would countain mismatch and would be unusable.
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