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How to determine concentration of phage-like particles?

MS2 bacteriophage phage-like particle concentration

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#1 Guf

Guf

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Posted 25 March 2013 - 04:07 AM

Hello,

I have a problem. I need to determinate concentration of MS2 phage-like particles (they do not form plaques). I found that some authors use correlation between absorbance, molecular weight and "extinction coefficient" to determine number of MS2 phage-like particles in the solution:

citation: The number of AR2 particles may be determined using the extinction coefficient of 1 OD260=
8 mg/ml of MS2 bacteriophage and the molecular weight is 3 x 106. Based on this procedure, approximately 1 x 1016 MS2 phage-like particles can be purrified from one liter of cultuire.

another citation: The concentration of MS2 phage-like particles was determined using an extinction coefficient of 8 mg/ml of MS2 bacteriophage per absorbance unit at 260 nm and a molecular weight of 3.0 x 106.

Please, what is the exact method of calculation, I'm not a mathematician. I need to know how many MS2 phage-like particles (103 or 106) are present in the solution. Could you give me an example of calculation, please? Thank you.





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