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Stain nitrocellulose membrane with coomassie stain?

nitrocellulose membrane coomassie stain

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3 replies to this topic

#1 kalindi

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Posted 24 March 2013 - 06:40 PM

Hey I m a researcher...I did one western blot transfer and my sir told me to stain nitrocellulose membrane with coomassie stain...but then disaster happen...stain was absobed by the membrane and now destaining is not possible...
So, can we stain membrane with coomassie stain??? Ponceau S is working well

#2 bob1

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Posted 25 March 2013 - 12:14 AM

Yes, you can stain membranes with coomassie, but it isn't reversible like ponceau S staining is. If you want to stain with coomassie, it is probably easier to do so in the gel.

#3 mdfenko

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Posted 25 March 2013 - 05:39 AM

if you haven't blocked the membrane prior to staining then you should be able to destain the coomassie stained membrane (not the protein bands). it may take some time and changes of destaining solution but you should be able to clear the background (i've done it).
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#4 ajithnandanam

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Posted 28 March 2013 - 04:46 AM

ponceau S works fine and it gets removed easily by washing with water.





Also tagged with one or more of these keywords: nitrocellulose membrane, coomassie stain

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