Must the terminator be in frame?
Posted 23 March 2013 - 04:09 AM
What will happen?
The manuals for some commercial vectors, such as pET, say that the terminator isn't quite necessary. My vector is self-made and the promoter and terminator are specific.
- reurfassern likes this
Posted 23 March 2013 - 04:57 AM
There is no logical reason for it to be in frame afaik, since it's role is to to detach polymerase, RNApol doesn't care whether someting is in frame or not, ribosomes do care.
Furthermore, after stop codon, no "frame" longer exists actually.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon