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Nested PCR


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2 replies to this topic

#1 SarahLee8

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Posted 22 March 2013 - 10:18 AM

Hello,

I will be attempting a PCR and I was wondering how much of my reaction with my first primer set I should add to the second reaction. I've also read that an exonuclease can be added before the second reaction to eliminate anything that is left of the first primer set. Is this necessary?

Thank you.

#2 hobglobin

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Posted 22 March 2013 - 12:27 PM

have a look here: http://www.protocol-...osts/30427.html
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 SarahLee8

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Posted 25 March 2013 - 08:55 AM

Great, thank you for the link!




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