cell binding assay with nanoparticles?
Posted 21 March 2013 - 05:32 PM
our collaborators have sent me some nanoparticle they have made. they have conjugated it to an antibody we use for targeting certain cell types. I need to assay for functionality to be certain our antibody targeting is not hindered by addition of the nanoparticle.
our typical plate-based cell binding assays involve adding our antibody to cells (human lymphoma and/or leukemia cells), going through a few wash steps, and assaying for remaining antibody (unbound Ab will be removed with supernatant when cells are pelleted).
well, the NP is very dense and can't be washed away. any spin that will pellet out cells will also pellet out Ab.
the NP is also magnetic, but I don't know a way to clearly pull the cells-Ab-NP complex out and quantify binding.
does anyone have any suggestions?
Posted 22 March 2013 - 12:27 AM
Posted 22 March 2013 - 10:55 AM