Is there anyone who's working with Callosobruchus Maculatus? I'm having trouble culturing Callosobruchus. They always die.
Can anyone tell me your successful culture conditions, such as temperature, humidity, the way you collect embryos and maintain the population?
Submit your paper to J Biol Methods today!
Is there anyone who's working with Callosobruchus Maculatus?
Started by Honghu, Mar 21 2013 04:46 PM
Callasobruchus Culture Bean Beetle
2 replies to this topic
#2
hobglobin
-
- Global Moderators
-
- 5,465 posts
Growing old is mandatory, growing up is optional...
90
Excellent
Excellent
Posted 22 March 2013 - 06:50 AM
So far I never reared them but successful culture methods are available online: http://www.beanbeetl...rg/handbook/#CT
Anyway the reasons for a unsuccessful rearing can be a lot: inbreeding, wrong food, contaminated food (pesticides), wrong environmental conditions, diseases, etc. Also several reasons can act together of course. Especially the contaminated food is an often overlooked and serious problem when you don't have access to "clean" products, as insecticides have sometimes surprisingly long persistence times and some products (depending on origin) also high loads...
Anyway the reasons for a unsuccessful rearing can be a lot: inbreeding, wrong food, contaminated food (pesticides), wrong environmental conditions, diseases, etc. Also several reasons can act together of course. Especially the contaminated food is an often overlooked and serious problem when you don't have access to "clean" products, as insecticides have sometimes surprisingly long persistence times and some products (depending on origin) also high loads...
Edited by hobglobin, 22 March 2013 - 07:09 AM.
- Honghu likes this
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#3
Honghu
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 22 March 2013 - 03:14 PM
Thanks! We are using the organic black eye peas right now, so I guess the food is not an issue. Although I'm doubting this may result from the high humidity (more than 60%) at 30 degree incubator. I'll do an experiment to figure out next week.
Also tagged with one or more of these keywords: Callasobruchus, Culture, Bean Beetle
Protocols and Techniques Forums →
General Lab Techniques →
Do S2 cells normally have small 1-2um objects floating in culture?Started by fruitfly1, 07 Feb 2014  culture, drosophila, cell, S2 and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Lymphocyte Cell LineStarted by jerryshelly1, 16 Jan 2014 lymphocyte, cell line, culture
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Macrophage isolation - strange debrisStarted by soho, 06 Dec 2013 macrophage, debris, isolation and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Preparing a cell culture stock to plate out at 7x10^5 per well?Started by Frazermb, 07 Nov 2013 THP-1, culture, cell count, stock and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
Monocyte culture helpStarted by sryu4, 23 Jul 2013 monocyte, culture, time, rat and 1 more...
|
|
|
