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How to measure reactive oxygen species using a specific fluorescent dye/indicato

ROS reactive oxygen species fluorescent ROS measure

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#1 NeuroADResearch

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Posted 21 March 2013 - 01:36 PM

When a fluorescent dye/indicator is added to a cell culture to show the ROS in the culture, how can the amount of reactive oxygen species be estimated or determined? Is there an easy method, possibly not costly?

Thanks for the responses!

#2 mdfenko

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Posted 22 March 2013 - 04:48 AM

you can determine relative fluorescence of the culture medium and compare to a standard curve.
talent does what it can
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#3 andromeda

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Posted 02 April 2013 - 08:38 AM

I am not sure if its of any help but the following article might be useful it's called:
Measurement of Reactive Oxygen Species in the Culture Media Using Acridan Lumigen PS-3 Assay
and the link is:
http://www.ncbi.nlm....les/PMC3165859/





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