When a fluorescent dye/indicator is added to a cell culture to show the ROS in the culture, how can the amount of reactive oxygen species be estimated or determined? Is there an easy method, possibly not costly?
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How to measure reactive oxygen species using a specific fluorescent dye/indicato
Started by NeuroADResearch, Mar 21 2013 01:36 PM
ROS reactive oxygen species fluorescent ROS measure
2 replies to this topic
#1
NeuroADResearch
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#2
mdfenko
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Posted 22 March 2013 - 04:48 AM
you can determine relative fluorescence of the culture medium and compare to a standard curve.
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#3
andromeda
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Posted 02 April 2013 - 08:38 AM
I am not sure if its of any help but the following article might be useful it's called:
Measurement of Reactive Oxygen Species in the Culture Media Using Acridan Lumigen PS-3 Assay
and the link is:
http://www.ncbi.nlm....les/PMC3165859/
Measurement of Reactive Oxygen Species in the Culture Media Using Acridan Lumigen PS-3 Assay
and the link is:
http://www.ncbi.nlm....les/PMC3165859/
Also tagged with one or more of these keywords: ROS, reactive oxygen species, fluorescent, ROS measure
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