When a fluorescent dye/indicator is added to a cell culture to show the ROS in the culture, how can the amount of reactive oxygen species be estimated or determined? Is there an easy method, possibly not costly?
Thanks for the responses!
Submit your paper to J Biol Methods today!
How to measure reactive oxygen species using a specific fluorescent dye/indicato
Started by NeuroADResearch, Mar 21 2013 01:36 PM
ROS reactive oxygen species fluorescent ROS measure
2 replies to this topic
#1
NeuroADResearch
-
- Active Members
-
- 14 posts
member
3
Neutral
Neutral
#2
mdfenko
-
- Active Members
-
- 3,242 posts
an elder
198
Excellent
Excellent
Posted 22 March 2013 - 04:48 AM
you can determine relative fluorescence of the culture medium and compare to a standard curve.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
andromeda
-
- Active Members
-
- 8 posts
member
2
Neutral
Neutral
Posted 02 April 2013 - 08:38 AM
I am not sure if its of any help but the following article might be useful it's called:
Measurement of Reactive Oxygen Species in the Culture Media Using Acridan Lumigen PS-3 Assay
and the link is:
http://www.ncbi.nlm....les/PMC3165859/
Measurement of Reactive Oxygen Species in the Culture Media Using Acridan Lumigen PS-3 Assay
and the link is:
http://www.ncbi.nlm....les/PMC3165859/
Also tagged with one or more of these keywords: ROS, reactive oxygen species, fluorescent, ROS measure
Protocols and Techniques Forums →
Protein and Proteomics →
Use of fluorescent stain on IEF gelsStarted by Missle, 15 Mar 2016  IEF, Fluorescent, fixation
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Fluorescently labelled primer probes for Northern blottingStarted by spongya77, 12 May 2014 Fluorescent, primer probes and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
fluorescent tag on membrane proteinStarted by mengkong2, 26 Feb 2014 fluorescent, cloning, plasmid
|
|
|
||
Protocols and Techniques Forums →
Cell Biology →
H2DCFDA for MDCK cellsStarted by rabraw7, 27 Oct 2013 ROS, MDCK, Confocal
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or negStarted by ImposterSyndrome, 03 Sep 2013 vector, fluorescent and 3 more...
|
|
|
