Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

SuperScript II RT instead of TaqMan® MicroRNA Reverse Transcription Kit

Started by smartyspb, Mar 20 2013 06:37 AM

miRNA cDNA Reverse Transcription qRT-PCR

Please log in to reply

No replies to this topic

#1 smartyspb

member

Members
1 posts

0
Neutral

Posted 20 March 2013 - 06:37 AM

Dear colleagues,

I am wondering if someone used SuperScript II RT for miRNA instead of recommended TaqMan kit for the further qRT-PCR quantification of miRNA?

Does anybody have idea how SuperScript can influence the synthesis of cDNA of miRNA?

Thank you in advance!

Back to siRNA, microRNA and RNAi · Next Unread Topic →

Also tagged with one or more of these keywords: miRNA, cDNA, Reverse Transcription, qRT-PCR

Protocols and Techniques Forums → siRNA, microRNA and RNAi → Detecting miRNA mimics by RT-PCR? Started by Guest_SD1_* , 02 May 2013 miRNA, RT-PCR	4 replies 195 views	SD1 08 May 2013
Protocols and Techniques Forums → siRNA, microRNA and RNAi → Overexpressing miR - rescue by co-overexpressing its target Started by Guest_PBT_* , 31 Mar 2013 miRNA, microRNA, overexpression and 2 more...	0 replies 305 views	PBT Yesterday, 11:18 PM
Protocols and Techniques Forums → Molecular Biology → What is the necessary time for lipofectamin to make complex with pre-miRNA oligo Started by Guest_gingerx_* , 19 Mar 2013 transfection, miRNA	1 reply 171 views	bob1 20 Mar 2013
Protocols and Techniques Forums → siRNA, microRNA and RNAi → miRNA quantification Started by Guest_dude3005_* , 18 Mar 2013 qPCR, reverse transcription and 1 more...	3 replies 331 views	imoani 26 Mar 2013
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → Steps in performing qRT-PCR for microarray validation Started by Guest_Neanderthal_* , 06 Mar 2013 microarray validation and 4 more...	0 replies 129 views	Neanderthal 06 Mar 2013