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SuperScript II RT instead of TaqManŽ MicroRNA Reverse Transcription Kit

miRNA cDNA Reverse Transcription qRT-PCR

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#1 smartyspb

smartyspb

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Posted 20 March 2013 - 06:37 AM

Dear colleagues,

I am wondering if someone used SuperScript II RT for miRNA instead of recommended TaqMan kit for the further qRT-PCR quantification of miRNA?

Does anybody have idea how SuperScript can influence the synthesis of cDNA of miRNA?

Thank you in advance!






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