random primers or oligodT
Posted 20 March 2013 - 01:05 AM
I checked my 12 genes of interest on cancer cell lines whose RNA was extracted using olidodT primers. I would ideally like to use oligodT primers for my RT-PCR in the clinical tissues, but the kits are very expensive. The cheaper kits use random primers.
Posted 20 March 2013 - 03:39 AM
Posted 20 March 2013 - 07:07 AM
Posted 20 March 2013 - 01:55 PM
I'm not even sure if you need to buy kits, we were doing classic RT with separate components, so you can then buy what primers you want.
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