Hi
I just transfected some cells with pre-miRNA oligos alone without any marker
The point forgot I did not wait 20minutes after adding lipo to plasmid solution.Just3 to 5 minutes after mixing lipofectamin with pre-mirs
is such matter really affects experiment this time?do cells absorb naked dsRNA in case of not having completely formed lipofectamin particles?
I was really disappointed while doing this assay because I do not see the desired effect on protein after several experiments and I do not have any method in my mind to check the transfection effeciency and thats such a disaster to wait after doing western to see what has happened
thanks everybody
0
What is the necessary time for lipofectamin to make complex with pre-miRNA oligo
Started by gingerx, Mar 19 2013 09:01 AM
transfection miRNA
1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 20 March 2013 - 12:18 AM
The time is important, 3-5 min is probably not long enough. Cells shouldn't take up the RNA without the transfection reagent.
Also tagged with one or more of these keywords: transfection, miRNA
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