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What is the necessary time for lipofectamin to make complex with pre-miRNA oligo

transfection miRNA

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#1 gingerx



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Posted 19 March 2013 - 09:01 AM

I just transfected some cells with pre-miRNA oligos alone without any marker
The point forgot I did not wait 20minutes after adding lipo to plasmid solution.Just3 to 5 minutes after mixing lipofectamin with pre-mirs
is such matter really affects experiment this time?do cells absorb naked dsRNA in case of not having completely formed lipofectamin particles?
I was really disappointed while doing this assay because I do not see the desired effect on protein after several experiments and I do not have any method in my mind to check the transfection effeciency and thats such a disaster to wait after doing western to see what has happened
thanks everybody

#2 bob1


    Thelymitra pulchella

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Posted 20 March 2013 - 12:18 AM

The time is important, 3-5 min is probably not long enough. Cells shouldn't take up the RNA without the transfection reagent.

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