Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What is the necessary time for lipofectamin to make complex with pre-miRNA oligo

transfection miRNA

  • Please log in to reply
1 reply to this topic

#1 gingerx

gingerx

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 19 March 2013 - 09:01 AM

Hi
I just transfected some cells with pre-miRNA oligos alone without any marker
The point forgot I did not wait 20minutes after adding lipo to plasmid solution.Just3 to 5 minutes after mixing lipofectamin with pre-mirs
is such matter really affects experiment this time?do cells absorb naked dsRNA in case of not having completely formed lipofectamin particles?
I was really disappointed while doing this assay because I do not see the desired effect on protein after several experiments and I do not have any method in my mind to check the transfection effeciency and thats such a disaster to wait after doing western to see what has happened
thanks everybody

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,710 posts
398
Excellent

Posted 20 March 2013 - 12:18 AM

The time is important, 3-5 min is probably not long enough. Cells shouldn't take up the RNA without the transfection reagent.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.