There are some commercial miRNA quantification kits available (Qiagen, Exiqon, ABI etc) Are there any specific reasons to prefer one commercial miRNA quantification assay over the other? What are your experiences?
1
3 replies to this topic
#2
aimikins
-
- Global Moderators
-
- 112 posts
Sunflower
2
Neutral
Neutral
Posted 18 March 2013 - 09:47 AM
I do not work with miRNAs myself, but have you tried to use google for product reviews? have you checked your kits in biocompare.com?
"it is a miracle that curiosity survives formal education" -A.E.
#4
imoani
-
- Members
-
- 1 posts
member
0
Neutral
Neutral
Posted 26 March 2013 - 09:34 PM
Hi dude3005,
This article discusses different RNA isolation methods (with a section on miRNA): Eldh et al (2012), Molecular Immunology 50, 578-286.
McDonald et al (2011), Clinical Chemistry 57, 833-840 recommend Ambion's mirVana Paris kit for their serum/plasma samples.
We have just placed an order for the Exiqon isolation kit, mainly because we are profiling miRNA from serum and want to use Exiqon's LNA method, which is supposed to be more sensitive for low abundant targets.
Previously we have used Qiagen's miRNeasy kit which was quite good for our serum samples, but we haven't done a direct comparison of the different methods.
This article discusses different RNA isolation methods (with a section on miRNA): Eldh et al (2012), Molecular Immunology 50, 578-286.
McDonald et al (2011), Clinical Chemistry 57, 833-840 recommend Ambion's mirVana Paris kit for their serum/plasma samples.
We have just placed an order for the Exiqon isolation kit, mainly because we are profiling miRNA from serum and want to use Exiqon's LNA method, which is supposed to be more sensitive for low abundant targets.
Previously we have used Qiagen's miRNeasy kit which was quite good for our serum samples, but we haven't done a direct comparison of the different methods.
Also tagged with one or more of these keywords: qPCR, reverse transcription, miRNA
 |
Protocols and Techniques Forums →
Tissue and Cell Culture →
Housekeeper for HEK cells?Started by Guest_Stephp_* , 17 May 2013  HEK cells, house keeper, PCR and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
Detecting miRNA mimics by RT-PCR?Started by Guest_SD1_* , 02 May 2013 miRNA, RT-PCR
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
FAM and VIC S-curvesStarted by Guest_squallweathered_* , 23 Apr 2013 qPCR, FAM, VIC, DNA, RNA
|
|
|
|
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
Overexpressing miR - rescue by co-overexpressing its targetStarted by Guest_PBT_* , 31 Mar 2013 miRNA, microRNA, overexpression and 2 more...
|
|
|
||
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
SuperScript II RT instead of TaqManŽ MicroRNA Reverse Transcription KitStarted by Guest_smartyspb_* , 20 Mar 2013 miRNA, cDNA and 2 more...
|
|
|
