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miRNA quantification

qPCR reverse transcription miRNA

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3 replies to this topic

#1 dude3005

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Posted 18 March 2013 - 03:31 AM

There are some commercial miRNA quantification kits available (Qiagen, Exiqon, ABI etc) Are there any specific reasons to prefer one commercial miRNA quantification assay over the other? What are your experiences?

#2 aimikins

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Posted 18 March 2013 - 09:47 AM

I do not work with miRNAs myself, but have you tried to use google for product reviews? have you checked your kits in biocompare.com?
"it is a miracle that curiosity survives formal education" -A.E.

#3 dude3005

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Posted 19 March 2013 - 06:06 AM

yes!

#4 imoani

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Posted 26 March 2013 - 09:34 PM

Hi dude3005,

This article discusses different RNA isolation methods (with a section on miRNA): Eldh et al (2012), Molecular Immunology 50, 578-286.

McDonald et al (2011), Clinical Chemistry 57, 833-840 recommend Ambion's mirVana Paris kit for their serum/plasma samples.

We have just placed an order for the Exiqon isolation kit, mainly because we are profiling miRNA from serum and want to use Exiqon's LNA method, which is supposed to be more sensitive for low abundant targets.

Previously we have used Qiagen's miRNeasy kit which was quite good for our serum samples, but we haven't done a direct comparison of the different methods.





Also tagged with one or more of these keywords: qPCR, reverse transcription, miRNA

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