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what is the ORFeome clone?

ORF cloning

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#1 lna

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Posted 17 March 2013 - 08:25 PM

Dear all,
please, forgive me for this awkward question.
I want to use the desired gene (smothened protein) for cloning and the source of the ORF, which is available for me right now is described on this page:
http://www.kazusa.or...cgi?id=ORH09959
but fro; the information provided I can not really understand what type of DNA is this - is it the plasmid with the ORF insert or is it just linear ORF sequence?
Is anyone familiar with this kazasa source here?
Will strongly appreciate any help, thanks!

#2 bob1

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Posted 18 March 2013 - 12:14 AM

It appears to be a cDNA clone in a pENTR plasmid.

#3 lna

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Posted 18 March 2013 - 04:45 PM

It appears to be a cDNA clone in a pENTR plasmid.

thanks!





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