Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How viable is cell culture for a model?


  • Please log in to reply
2 replies to this topic

#1 Ubiquitous

Ubiquitous

    member

  • Active Members
  • Pip
  • 14 posts
1
Neutral

Posted 17 March 2013 - 11:33 AM

I'm sure many of you on here have seen this paper:

http://www.ncbi.nlm....pubmed/16697959

If adding serum to culture media causes cancer cells to terminally differentiate into lines that are completely different from their in vivo human counterparts, why do we still add serum then to media to grow cancer cells? This has been known since 2006, so why do we still continue to do it if the accuracy of such models is completely off from what would be expected to occur in vivo?

Are there more studies out there that have looked at whole transcriptomes of other commonly used cell lines that are purchased from vendors that also compared those transcriptomes to cells from a primary tumor? Just curious to see how much cell culture changes the in vivo pheno/genotypes, and if it is a reason why so much science fails when it comes time to translating research into the clinic.

#2 Tabaluga

Tabaluga

    Making glass out of shards

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 379 posts
48
Excellent

Posted 17 March 2013 - 01:37 PM

An interesting point to raise.
I think by using serum-free medium containing these growth factors one actually selects for the growth cancer stem cells, thereby enriching them in culture, although it mustn't be forgotten that these kind of cultures are only "enriched" for them, and do contain loads of more differentiated progeny and even some terminally differentiated cells.
I guess a lot of it is due to the great difference in availability, isn't it ? Everyone can get their hands on the established cell lines, while getting primary cells is more difficult.
And taking normal cell lines and just culturing them in EGF+/FGF+/serum- medium won't solve the problem, in my opinion. Take glioblastoma as an example - there are so many papers out nowadays where they take common cell lines like U87 for instance, grow them in said medium and make experiments with the spheroids as "glioblastoma stem cells". I think you really have to use primary cells for that.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 rhombus

rhombus

    Rhombus/Uncle Rhombus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 214 posts
20
Excellent

Posted 18 March 2013 - 08:39 AM

Serum is just one problem, what about:-

Genetic Drift

Immortalisation

21% Oxygen growing conditions ???????( tissue oxygenation)

Mycoplasma contaminations

Cryptic contaminations

Etc Etc Etc

Kindest regards

Uncle Rhombus




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.