study of receptors in cell lines
Posted 15 March 2013 - 01:29 AM
I'm styudying receptor in cancer cell lines. Since it is overexpressed I plan to observe the effect of a receptor antagonist in the cancer cell lines (e.g. MTT by proliferation). My problem is that the antagonist that's available may not be 100% selective to the recetor I'm studying (e.g receptor A) and that could also affect receptor B as well.
How do I ensure that the B receptor -selective antagonists are not active in my assay to have more compelling results.
I was thinking I treat the cell lines with antagonist to receptor A alone, antagonist to receptor B alone and combination of both antagonists of A and B?
Posted 15 March 2013 - 03:53 AM
after this you can go into the functional assays dealing prolfieration, survival, etc etc....,based on the biological activity of your target receptor....
I always had an alternate hypothesis....