Ponceau red problem: membrane turned red
Posted 14 March 2013 - 11:34 AM
I've done Ponceau staining of my PVDF membrane after the transfer and got a lot of background binding that doesn't go away after washing with water. My whole membrane is red, with transferred bands visible on that red background. Does it mean there is no point continuing with this membrane? Anyone can explain why on earth did it happen?
Posted 14 March 2013 - 11:40 AM
Try washing the membrane in a protein containing solution (e.g. 3% non-fat milk powder in TBS-t).
Posted 14 March 2013 - 11:58 AM
I always had an alternate hypothesis....