Maintainence of transformed cells
Posted 14 March 2013 - 07:38 AM
I am new at clonning but I have obtain (not without a lot of problems ;P) transformed cells with my insert of interest. Right now I have this clones in an agar plate and now I want to prepare cultures with grlycerol and freeze them. If this LB plates are a little old is it possible that the cells have lose the vector?
The other question is if it is better to prepare a new plate with isolated colonies and freeze a unique clon or I can pick a group of colonies of my master plate and grow in liquid LB to freeze.
Thanks you very much in advance for your help
Posted 14 March 2013 - 08:45 AM
Posted 14 March 2013 - 09:28 AM
Posted 14 March 2013 - 10:23 AM
Posted 14 March 2013 - 11:43 AM