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Differentiation of L6 myocytes

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#1 buzzi



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Posted 05 February 2004 - 07:13 AM

we need to measure insulin dependent glucose uptake in L6 myotubes. In our first experiments we saw no insulin dependent response ( however reasonably ok for adipocytes). we believe its due to the uncomplete differentiation. has anyone experience how to optimise that. should we subclone and if so how.

Edited by buzzi, 05 February 2004 - 07:13 AM.

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