My GST-tagged protein has a Factor Xa site between the tag and the protein. In order to optimise conditions I have tried incubating 0.5U to 10U of Factor Xa with 5uM of my protein for time periods of 2-16 hrs. But when I run the samples on SDS-PAGE, I do not see anything. The gel looks like there was absolutely no protein loaded. I am unsure as to what is going on. Please help.
Edited by Kmu, 13 March 2013 - 04:50 PM.