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Mouse genotyping issues


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#1 LacquerHead

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Posted 13 March 2013 - 02:59 AM

Hi all,

Wondering if you can help. I am having some issues with genotyping, using a traditional KO LacZ gene targeting strain - and although I get exactly the predicted bands for LacZ, WT and both for het that the original paper predicted I am at times still seeing protein and transcripts of the knocked out protein in the tissue of a KO mouse. I am wondering if years of inbreeding may have introduced some mutation, not really sure how to test this. Any ideas would really help, thanks!

#2 bob1

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Posted 13 March 2013 - 11:29 AM

Functional pseudogene? Is it possible that the KO was a not a full length KO?

Spontaneous mutations do happen, but they are relatively rare, and it is unlikely that it would return a gene that has been removed. Perhaps there was a recombination event in the original mouse that resulted in the KO being only one allele?




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