Blank spots on WB after semi-dry, NO BUBBLES
Posted 12 March 2013 - 09:38 AM
I transfer at 100 mA for 40 minutes for a mini-gel in a Fisher semi-dry blot unit. I'm slightly weighing the top of the blotter down with an ice bucket since the sandwich is too thick to tighen the knobs.
Where there are no blank spots - the transfer looks great.
Posted 12 March 2013 - 04:32 PM
Only advice I have is try another labs semi-dry rig or reduce the amount of watmans you use in your sandwhich. You should be able to fully close the appartus, you should not place an ice bucket on top. This is going to interfere with you electrodes forming a closed circuit.
Posted 13 March 2013 - 06:41 AM
Posted 13 March 2013 - 07:06 AM
you may need to degas your transfer buffers prior to wetting the filter papers and soaking the gel.
it could be that your filter papers are too wet.
it could be from insufficient removal of sds from the gel during the pre-transfer soak.
it could be happening because of insufficient pressure on the sandwich.
why are your sandwiches too thick to properly close the apparatus?
Edited by mdfenko, 13 March 2013 - 07:09 AM.
genius does what it must
i do what i get paid to do