In solution digestion
Posted 12 March 2013 - 06:42 AM
I am doing my in solution digestion with trypsine. And this is my pilot study with BSA.
According to optimal pH for trypsine activity I have tried to adjust the solution pH to 8-8.5. Usually I use ammonium bicarbonate 50mM for adjusting. but this time I use Ammonia. Now I want to know if it will work or the ammonia will have some effect on the procedure and also for peptides?
Posted 12 March 2013 - 04:41 PM
Edit: reference. Unfortunately, this is the only reference I can find. I know I remember reading about ammonia forming ammonia salts following amino acid cleavage (probably in voet and voet).
Edited by jerryshelly1, 12 March 2013 - 04:43 PM.