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quick change mutagenesis......


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#1 kartiga natarajan

kartiga natarajan

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Posted 12 March 2013 - 06:29 AM

Can anyone of u upload ur gel showing the PCR product before and after Dpn1 digestion? So that it will be helpful.....

#2 GNANA

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Posted 12 March 2013 - 07:57 AM

i dnt hav the habit of storing the PCR images, anyways, you shd see a single band though not much revealing...sometimes you ll see a smear around or over the product. after digestion when you run you still see the same band but a bit more intense smear...

Note: i always run the whole product before and after digestion, subsequently i purify it.
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




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