How to create a fully methylated control?
Posted 12 March 2013 - 03:59 AM
Do you know any way to have a truly completely methylated control, independently from the species?
Thanks a lot,
Posted 12 March 2013 - 04:09 AM
Edited by lmg, 12 March 2013 - 04:10 AM.
Posted 12 March 2013 - 04:18 AM
I'm not working with human and I need to have a DNA from my species (2 different fish). Any other idea?
Posted 12 March 2013 - 10:15 AM
Posted 25 August 2013 - 11:27 PM
Hi, most methylated DNAs from commercial parties are not entirely methylated.
A friendly lab once tried with replacing the Cytosine base in dNTP with 5-methylcytosine and doing a whole genome amplification reaction.
the are still using it and generally happy.
Also an excelent way to make fully unmethyled DNA, do a whole genome amplification using normal cytosine when making your dNTP mixture.
the kit they used was officially for human but it worked for their insect species. maybe order a sample at a supplyer and give it a go?