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methylation specific PCR (MSP)


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#1 mario2004

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Posted 04 February 2004 - 12:49 AM

I know lot of people doing methylation research use MSP for methylation mapping. MSP depends on 1-2 bases in the primer to discriminate 5mC or non 5mC. I wonder how is reproducibilty of MSP since once I made a primer with a wrong base at the very 3' end and I could still got amplification of expected product. If you do 3 MSP, what is the chance of getting the same result?

than you for any input.

mario

#2 labrat

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Posted 10 February 2004 - 08:07 PM

I once asked a similar question to people from the lab which invented the MSP technique and was told it works well at their hands. I think you have to tweak PCR conditions such as Ta, number of cycles. Unless you got them all optimized, this is a big concern.




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