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HeLa growing cloudy

HeLa

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4 replies to this topic

#1 chandra3316

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Posted 10 March 2013 - 09:54 PM

I am reviving Hela, I don't know why( the cells after the medium change on second day..)in third day or later looking to be dusty..some dark precipitation like thing in the cell. please reply soon.
( the cell line are one month old stored at -80; we use DMEM medium)

#2 GNANA

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Posted 10 March 2013 - 11:23 PM

cloudy and dusty, sounds your cells are contaminated.....just throw it off and start with new vial...change medium, trypsin and PBS when you take the new vial...it is advisable to sterilize the incubator as well.
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

#3 Tabaluga

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Posted 11 March 2013 - 12:21 AM

Agree, "cloudy" always sounds like a contamination....

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#4 chandra3316

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Posted 12 March 2013 - 04:18 AM

I am sure it is not contamination.. Mostly because of maintaining conditions...I guess I Used lot of spirit to clean my hands before placing it in the incubator. so it caused the stress to them..:) what do you say??
please discuss possibility for this or high medium or thawing(DMSO)....)

#5 Tabaluga

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Posted 12 March 2013 - 04:50 AM

can you post a picture ?
could it be that it's just a lot of cell debris ?

Edited by Tabaluga, 12 March 2013 - 12:01 PM.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 






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