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qPCR multiplex- impact of the fluorophore on probe annealing temperature


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#1 dan_

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Posted 08 March 2013 - 09:33 AM

Hi all,

Does anyone knows if the annealing temperature of probes is altered if an fluorophore and/or a quencher is link to the probe? Does all fluorophore has the same impact?

Thanks

#2 jerryshelly1

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Posted 10 March 2013 - 08:16 PM

Run a PCR and check




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